Bago Jurič, Ivona and Plečko, Vanda and Anić, Ivica and Pleško, Sanja and Jakovljević, Suzana and Rocca, Jean Paul and Medioni, Etienne (2015) Antimicrobial efficacy of photodynamic therapy, Nd:YAG laser and QMiX solution against Enterococcus faecalis biofilm. = Antimicrobial efficacy of photodynamic therapy, Nd:YAG laser and QMiX solution against Enterococcus faecalis biofilm. Photodiagnosis and photodynamic tehrapy, 1. pp. 1-5. ISSN 7891-352X. Vrsta rada: ["eprint_fieldopt_article_type_article" not defined]. . Točan broj autora: 7.
Full text not available from this repository.Abstract
Lasers have been recommended in final root canal disinfection protocol, however, there is no clear evidence about their efficacy against bacteria in biofilms. The aim of the study was to evaluate and compare the disinfection effect of antimicrobial photodynamic therapy (aPDT), Nd:YAG laser and QMiX solution against Enterococcus faecalis biofilm. METHODS: The study sample consisted of 65 dentine slices, which were inoculated with E. faecalis and incubated anaerobically for three weeks. The dentine discs were randomly allocated to one of the following experimental groups: aPDT (100 mW, 10mg/ml phenothiazinium chloride, 1 min), Nd:YAG laser (2 W, 15 Hz, 4x5s), QMiX solution (1 min). Positive controls did not receive any treatment and negative controls were treated with 5.25% NaOCl. To harvest surviving adherent cells, each dentine sample was transffered to a test tube containing of TSB, serial ten-fold dilutions were made and aliquot of 1 ml was plated onto blood agar plates and incubated for 48 h. Colony forming units grown were counted and transformed into actual counts based on the dilution factor. The remaining viable cells after each protocol were analysed by FISH. RESULTS: The aPDT and the QMiX solution were equally effective, with the reduction rate of E. faecalis CFUs of 98.8% and 99.3% respectively (p=1.107). The Nd:YAG laser caused 96% reduction of E. faecalis (P<0.001). CONCLUSION: The aPDT and the QMiX solution showed similar antibacterial efficacy against old E. faecalis biofilm, followed by Nd:YAG irradiation.
| Item Type: | Article (["eprint_fieldopt_article_type_article" not defined]) |
|---|---|
| Keywords (Croatian): | laser, root canal, bacteria, photodynamic therapy |
| Subjects: | BIOMEDICINE AND HEALTH > Dental medicine |
| Divisions: | 1000 Department of Materials > 1010 Chair of Materials and Tribology |
| Date Deposited: | 21 Sep 2016 13:37 |
| Last Modified: | 21 Sep 2016 13:37 |
| URI: | http://repozitorij.fsb.hr/id/eprint/6765 |
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