Cellular uptake and toxicity effects of silver nanoparticles in mammalian kidney cells

Milić, Mirta and Leitinger, Gerd and Pavičić, Ivan and Zebić Avdičević, Maja and Dobrović, Slaven and Goessler, Walter and Vinković Vrček, Ivana (2015) Cellular uptake and toxicity effects of silver nanoparticles in mammalian kidney cells. = Cellular uptake and toxicity effects of silver nanoparticles in mammalian kidney cells. JAT. Journal of applied toxicology, 35 (6). pp. 581-592. ISSN 0260-437X. Vrsta rada: ["eprint_fieldopt_article_type_article" not defined]. Kvartili JCR: Q2 (2015). Točan broj autora: 7.

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Abstract

The rapid progress and early commercial acceptance of silver-based nanomaterials is owed to their biocidal activity. Besides embracing the antimicrobial potential of silver nanoparticles (AgNPs), it is imperative to give special attention to the potential adverse health effects of nanoparticles owing to prolonged exposure. Here, we report a detailed study on the in vitro interactions of citrate-coated AgNPs with porcine kidney (Pk15) cells. As uncertainty remains whether biological/cellular responses to AgNPs are solely as a result of the release of silver ions or whether the AgNPs themselves have toxic effects, we investigated the effects of Ag+ on Pk15 cells for comparison. Next, we investigated the cellular uptake of both AgNPs and Ag+ in Pk15 cells at various concentrations applied. The detected Ag contents in cells exposed to 50mgl1 AgNPs and 50mgl1 Ag+ were 209 and 25 μg of Ag per 106 cells, respectively. Transmission electron microscopy (TEM) images indicated that the Pk15 cells internalized AgNPs by endocytosis. Both forms of silver, nano and ionic, decreased the number of viable Pk15 cells after 24 h in a dose- dependent manner. In spite of a significant uptake into the cells, AgNPs had only insignificant toxicity at concentrations lower than 25mgl1, whereas Ag+ exhibited a significant decrease in cell viability at one-fifth of this concentration. The Comet assay suggested that a rather high concentration of AgNP (above 25mgl1) is able to induce genotoxicity in Pk15 cells. Further studies must seek deeper understanding of AgNP behavior in biological media and their interactions with cellular membranes.

Item Type:	Article (["eprint_fieldopt_article_type_article" not defined])
Keywords (Croatian):	silver nanoparticles; Pk15 cells; absorption; cytotoxicity; genotoxicity
Subjects:	BIOMEDICINE AND HEALTH > Public health and health care
Divisions:	500 Department of Energy, Power Engineering and Environment > 540 Chair of Water and Environmental Engineering
Indexed in Web of Science:	Yes
Indexed in Current Contents:	Yes
Quartiles:	Q2 (2015)
Date Deposited:	27 Feb 2017 12:00
Last Modified:	05 Jun 2017 14:25
URI:	http://repozitorij.fsb.hr/id/eprint/7469

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